pyrAB [2019-08-27 12:04:51]

carbamoyl-phosphate synthetase (catalytic subunit)

Locus: BSU_15520

Isoelectric point: 4.78

Molecular weight: 117.44 kDa

Protein length: 1071 aa

Gene length: 3216 bp

Function: pyrimidine biosynthesis

Product: carbamoyl-phosphate synthetase (catalytic subunit)

Essential: no

E.C.: 6.3.5.5

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,623,736 → 1,626,951

The protein

Catalyzed reaction/ biological activity

• 2 ATP + H₂O + hydrogencarbonate + L-glutamine --> 2 ADP + carbamoyl phosphate + 2 H⁺ + L-glutamate + phosphate (according to UniProt)

Protein family

• carB family (with CarB, according to UniProt)

Paralogous protein(s)

• CarB

Structure

• 1JDB (from Escherichia coli, 49% identity, 69% similarity) PubMed

Localization

• membrane PubMed

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

Biological materials

Mutant

• BKE15520 (ΔpyrAB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GATTACTAAAATTTTGTTAA, downstream forward: _UP4_AATCAGGAGGCGGCAGTCAC
• BKK15520 (ΔpyrAB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GATTACTAAAATTTTGTTAA, downstream forward: _UP4_AATCAGGAGGCGGCAGTCAC

References

Reviews

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Original publications

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